Wine Cap Mushroom Spawn: How To Grow Wine Cap Mushrooms?
The wine cap mushroom is one of the most popular edible fungi. They are found in many parts of the world and they have been used medicinally for centuries. There are two species of wine cap mushrooms, Psilocybe cubensis and Psilocybe semilanceata. Both species grow under different conditions but both species produce spores which can be grown under certain circumstances (see table).
In order to grow wine cap mushrooms indoors, you need to obtain a few things first. You will need a wine bottle or other container with a tight fitting lid. You will also need some white plastic film or paper towels, some bleach and hot water. If you don’t have any of these items already, then you can buy them from your local grocery store.
Step 1: Prepare Your Container
You will need a container with a tight fitting lid. A wine bottle works well because it is easy to clean and sterilize. You can use any type of container that fits tightly around the inside of the bottle without leaking air out. A glass jar works too, but you may want to make sure there isn’t enough room inside for bacteria or mold growth if you choose this option.
Next, you need to make holes in the bottom of the container. The holes will let water and air escape as well as letting the mushrooms grow through. The more holes, the less likely that there will be bacteria or mold growth inside the container. Make ten to twenty holes in a circle around the bottom.
Use a nail or some other sharp object to poke holes around the bottom of your container.
Step 2: Prepare The Substrate
The next step is to fill your container with a growing medium. You can use Jiffy-Mix, seed starting mix, or vermiculite if you want to grow your mushrooms on a substrate. If you want to cultivate them without a substrate, you can skip this step. The holes in the bottom of the container will allow the water to drain out, so no additional draining will be required.
Add water to the mix until it is the consistency of cookie dough.
Step 3: Prepare The Filter Material
The filter material will let solid matter like bits of mushroom grow through while blocking bacteria, mold, and other contaminants from getting through. Lay a sheet of white plastic film or paper towel on top of the growing medium. Wet it thoroughly with the hottest water you can get from your faucet. Allow the water to drain through for a few minutes.
The water will turn dark brown as it picks up dirt and other contaminants.
Step 4: Prepare The Spores
Get a fresh spore syringe or isolate from the culture you are working with. Get a sterile needle or scalpel and using aseptic technique, make several small injections in the spore syringe until it begins to turn yellowish brown. This is the color that signifies that enough spores have been released.
Step 5: Release The Spores
Take the spore syringe (or spore print if you don’t have a syringe) and stab it several times with the sterilized needle. The spores will be released into the water inside the syringe. Swirl it around to make sure that all of the spores are released into the solution and not stuck to the sides or bottom of the syringe.
Step 6: Inoculation
Once the filter material has drained, hold the container at eye level and gently pour the spore/water solution into the container until it is about halfway full. If you use a filter patch instead of a spore syringe, then simply release the spores onto the filter patch and then fold it in half and gently pour the spore water directly onto it. You want to make sure that the spores make direct contact with the moist filter paper.
Step 7: Incubation
It will take about four to ten days for the mycelium to reach maturity and then you can harvest the mushrooms. In the meantime, keep the container at room temperature in indirect sunlight. Never expose the container to sunlight, as this can scorch the developing mushrooms.
Step 8: Harvesting
Spore prints or live mycelium are the first stage of mushroom cultivation and these are edible. Once the mushrooms have grown to full maturity, they can be harvested. You can also let them grow further to see if you get a larger harvest. If you eat the mushrooms before they are mature, they may not have the desired full effects.
Step 9: Storage
You can dry the mushrooms and store them for later use. You can also eat them fresh or cook them for immediate consumption.
Tips & Warnings
You can use a used coffee filter in place of the plastic filter material.
Make sure that you work with clean equipment and wash your hands before handling the spore Syringe to avoid transferring bacteria that can harm or kill your cultures.
Do not inject water, alcohol, or anything other than spores into your spore syringe.
Make sure you buy a spore syringe that contains the desired spores. It may contain hundreds of different types of spores and bacteria.
Do not consume synthetic drugs or supplements before or after performing these procedures.
Spore prints and syringes can be obtained from many websites that sell mushroom related items.
Take care when working with knives, and always wash and sterilize your workspace to avoid any possible contaminants getting into your cultures.
Mushrooms are a type of fungus and are therefore not actually plants.
Make sure that you have stored the spore syringe or spore print in a refrigerator for no longer than a month before performing this procedure.
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Do not open the spore syringe until you are ready to use it.
Make sure that the spore syringe is kept out of the reach of children and pets.
Do not attempt to take spore prints from mushrooms in the wild without expert guidance. This is illegal in most places and can be very dangerous.
Spores require a moist environment to germinate, do not store them for long periods in dry conditions or they will die.
Make sure that you work in a well-ventilated area. Spores can be harmful if inhaled.
You can obtain spore syringes from many sources, but make sure that you are buying from a reputable dealer.
Do not expose the spore syringe to extreme cold, as this may kill the spores.
If your spore syringe is in a glass vial, you may need to boil it before use to sterilize it.
If you do not have access to a spore syringe, you can obtain spore prints from online forums and marketplaces. Spores in the wild can be dangerous. You may want to seek professional advice before collecting them yourself.
Making a spore syringe at home can be very simple or quite complex, depending on the equipment you have available. This procedure provides a safe and effective way for you to make your own spore syringes, without having special equipment.
The basic procedure involves sterilizing the spore print or spore syringe, then grinding it up into a liquid solution. Next, you inject this liquid into a liquid that contains no nutrients called “sterile water”. This allows the spores to form into mycelium, which is the root of the mushroom. This is then mixed with the sterilized substrate, which provides the nutrients for the mycelium to form into mushrooms.
The mushrooms will grow in a timely manner if the conditions are right and will provide a ready supply for home use or for selling.
The first step is to obtain a spore print or a spore syringe. If you are using a spore print, then you should obtain a fresh one from an online forum or marketplace. These can be obtained from many legal and safe sources, but make sure that you do not obtain one from anywhere illegal as this could land you in legal trouble. After obtaining your spore print,you should sterilize it.
To do this, take the spore print and place it into a container that has a lid with holes or some other kind of covering to allow air in but not out. Then place the container into the cooker and steam for at least thirty minutes. The spore should be completely sterilized after this. After the steaming process is done, take the spore print out and wait for it to cool down before handling.
The next step is to grind up the spore print into a fine powder. This can be done using a spoon, knife, or anything that can help crush it up into a paste like consistency. Since we want the mycelium to form roots and grow, the more surface area the better. The finer the grind, the faster this will happen.
If you have a mortar and pestle, this would be the ideal way to crush up the spore print.
Now, you need to mix the crushed spore print into a liquid that doesn’t contain any nutrients. The best way to do this is with sterile water, which can be purchased from online chemical suppliers or it may be available at your local pharmacy. Pouring the spore powder into the liquid while stirring is important so that the spores form into the liquid. If you are using sterile water, then one teaspoon of the powder is perfect for one milliliter of water.
If you have a gram scale, then each ml of sterile water should have roughly 1mg of spore powder.
After the sterile water has been mixed with the spore powder and stirred thoroughly, it needs to be injected into the liquid. To do this, take a plastic syringe from your local pharmacy or other source. It needs to be a sterile medical syringe that can handle at least 1ml of liquid. Check to make sure that the needle can also draw out the liquid.
If you are able to draw out at least 1ml, then it should work. After checking this, fill the syringe with the spore water mixed with the spore powder.
The next step is to sterilize the syringe using the cooker or industrial equivalent. To do this, fill the syringe with the spore water and place into the cooker. Bring up to temperature and keep it at 140 Celsius for at least twenty minutes or until you notice that the water has started to evaporate. If cooking isn’t possible, then you can try to use autoclaves, but these can be expensive and difficult to get unless you work in a medical or biological field.
After sterilizing the syringe, draw out 0.5 – 1ml and inject it into the honey. The deeper you inject, the better as this will allow the mycelium to grow more easily. Injecting it at a shallow depth will work as well, but not as good as deep injection.
Now that you have injected the spore solution into the honey, place it somewhere warm and dark. The best temperature is around 27-30 degrees Celsius, but any temperature above 20 degrees is fine. Within a few hours, you should see the mycelium start to grow and spread. G2G cloning will work but will be slower than if you were to use agar.
Once you have grown out the mycelium network (this can take a few days), it is now ready to be used. Take a knife or similar object and scrape off the mycelium so that it can be used for later. If you are using the mycelium as a inoculant, then the spores spread throughout the substrate will be enough to start the process. If you are using it for food, then you can either let the mycelium grow on whatever food it is able to or grind it into a fine powder using a mortar and pestle.
Now that the mycelium has been grown out, you can use it for various recipes.
Sources & references used in this article:
Polyporus umbellatus, an edible-medicinal cultivated mushroom with multiple developed health-care products as food, medicine and cosmetics: a review by AR Bandara, S Rapior, DJ Bhat, P Kakumyan… – Cryptogamie …, 2015 – BioOne
The complete mushroom hunter: An illustrated guide to finding, harvesting, and enjoying wild mushrooms by G Lincoff – 2010 – books.google.com
… Mushroom Hunter, Revised: Illustrated Guide to Foraging, Harvesting, and Enjoying Wild Mushrooms-Including new sections on growing your own incredible … by G Lincoff – 2017 – books.google.com
Wine tasting: a professional handbook by R Phillips, DA Reid – 2006 – Pan Macmillan
Quality and safety assurance in winemaking by RS Jackson – 2016 – books.google.com
Texas mushrooms: a field guide by T Christaki, C Tzia – Food Control, 2002 – Elsevier
Drinking and protecting: a market approach to the preservation of cork oak landscapes by C Orban – 2014 – SIU Press